Medical Studies

Dr Floyd H "Ski" Chilton’s Key Publications (Selected from Over 110).

Chilton FH, O'Flaherty JT, Walsh CE, Thomas MJ, Wykle RL, DeChatelet LR, Waite M. Platelet activating factor: Stimulation of the lipoxygenase pathway in polymorphonuclear leukocytes by 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine. J Biol Chem 257:5402-5407, 1982.

Walsh CE, DeChatelet LR, Chilton FH, Wykle RL, and Waite M. Mechanism of arachidonic acid release in human polymorphonuclear leukocytes. Biochim Biophys Acta 750:32-40, 1983.

Chilton FH, O'Flaherty JT, Ellis JM, Swendsen CL, Wykle RL. Metabolic fate of platelet activating-factor in neutrophils. J Biol Chem 258:6357-6361, 1983.

Chilton FH, O'Flaherty JT, Ellis JM, Swendsen CL, Wykle RL. Selective acylation of lyso platelet activating factor by arachidonate in human neutrophils. J Biol Chem 258:7268-7271, 1983.

Chilton FH, Ellis JM, Olson SC, Wykle RL. 1-O-alkyl-2-arachidonoyl-sn-glycero-3-phosphocholine: A common source of platelet-activating factor and arachidonate in human polymorphonuclear leukocytes. J Biol Chem 259:12014-12019, 1984.

Chilton FH, Murphy RC. Remodeling of arachidonate-containing phosphoglycerides within the human neutrophil. J Biol Chem 261:7771-7777, 1986.

Chilton FH, Hadley JS, Murphy RC. Incorporation of arachidonic acid into 1-acyl-2-lyso-sn-glycero-3-phosphocholine of the human neutrophil. Biochim Biophys Acta 917:48-56, 1987.

Swendsen CL, Chilton FH, O'Flaherty JT, Surles JR, Piantadosi C, Waite M, Wykle RL. Human neutrophils incorporate arachidonic acid and saturated fatty acids into separate molecular species of phospholipids. Biochim Biophys Acta 919:79-89, 1987.

Chilton FH, Murphy RC. Stimulated production and natural occurrence of 1,2- diarachidonoyl-glycerophosphocholine in human neutrophils. Biochem Biophys Res Commun 145:1126-1133, 1987.

Chilton FH, Connell TR. 1-ether-linked phosphoglycerides: major endogenous sources of arachidonate in the human neutrophil. J Biol Chem 263:5260-5265, 1988.

Chilton FH. Potential phosphoglyceride source(s) of arachidonate used for the synthesis of leukotrienes by the human neutrophil. Biochem J 258:327-333, 1989.

Chilton FH, Westcott JY, Zapp LM, Henson JE, Voelkel NF. Incorporation of arachidonic acid into cell lipids of the isolated perfused rat lung. J Appl Physiol 66(6):2763-2771, 1989.

Churchill L, Chilton FH, Resau JH, Bascom R, Hubbard WC, Proud D. Cyclooxygenase metabolism of endogenous arachidonic acid by cultured human tracheal epithelial cells. Am Rev Respir Dis 140:449-459, 1989.

Triggiani M, Chilton FH. Influence of immunologic activation and cellular fatty acid levels on the catabolism of platelet-activating factor within the murine mast cell (PT-18). Biochim Biophys Acta 1006:41-51, 1989.

Cluzel M, Undem BJ, Chilton FH. Release of platelet-activating factor and the metabolism of leukotriene B4 by the human neutrophil when studied in a cell superfusion model. J Immunol 143:3659-3665, 1989.

Chilton FH, Lichtenstein LM. Lipid mediators of the allergic reaction. In: Kimishige I, Lachmann PJ, Lerner R, Waksman BH, ed. Chemical Immunology. 49:173-205, 1990.

Undem BJ, Torphy TJ, Goldman DW, Chilton FH. Inhibition by adenosine 3:'5'-monophosphate of eicosanoid and platelet-activating factor biosynthesis in the mouse PT-18 mast cell. J Biol Chem 265:6750-6758, 1990.

Triggiani M, Hubbard WC, Chilton FH. Synthesis of 1-acyl-2-acetyl-sn-glycero-3-phosphocholine by an enriched preparation of the human lung mast cell. J Immunol 144:4773-4780, 1990.

Triggiani M, Connell TR, Chilton FH. Evidence that increasing the cellular content of eicosapentaenoic acid does not reduce the biosynthesis of platelet-activating factor. J Immunol 145:2241-2248, 1990.

Winkler, JD, Sung C-M, Bennett F, Chilton FH. Characterization of CoA-independent transacylase activity in U937 cells. Biochim Biophys Acta 1081:339-346, 1991.

Churchill L, Chilton FH, Proud D. Interaction of platelet-activating factor with cultured guinea pig tracheal epithelial cells. Biochem J 276:593-598, 1991.

Triggiani M, Schleimer RP, Warner JA, Chilton FH. Differential synthesis of 1-acyl-2-acetyl-sn-glycero-3-phosphocholine and platelet-activating factor by human inflammatory cells. J Immunol 147:660-666, 1991.

Triggiani M, Goldman DW, Chilton FH. Biological effects of 1-acyl-2-acetyl-sn-glycero-3-phosphocholine in the human neutrophil. Biochim Biophys Acta 1084:41-47, 1991.

Triggiani M, D'Souza DM, Chilton FH. Metabolism of 1-acyl-2-acetyl-sn-glycero-3-phosphocholine in the human neutrophil. J Biol Chem 266:6928-6935, 1991.

Cluzel M, Rohde CA, MacGlashan Jr. DW, Undem BJ, Chilton FH. Dynamics of platelet activating-factor-release and uptake in a human neutrophil suspension. Biochem J 278:453-459, 1991.

Nakamura T, Fonteh AN, Hubbard WC, Triggiani M, Inagaki N, Ishizaka T, Chilton FH. Arachidonic acid metabolism during antigen and ionophore activation of the mouse bone marrow-derived mast cell. Biochem Biophys Acta 1085:191-200, 1991.

Fonteh AN, Chilton FH. Rapid remodeling of arachidonate from phosphatidylcholine to phosphatidylethanolamine pools during mast cell activation. J Immunol 148:1784-1791, 1992.

Triggiani M, Fonteh AN, Chilton FH. Factors that influence the proportion of platelet- activating factor and 1-acyl-2-acetyl-sn-glycero-3-phosphocholine synthesized by the mast cell. Biochem J. 286:497-503, 1992.

O'Sullivan MG, Chilton FH, Huggins, JR EM, McCall CE. Lipopolysaccharide priming of alveolar macrophages for enhanced synthesis of prostanoids involves induction of a novel prostaglandin H synthase. J Biol Chem 267:14547-14550, 1992. (This is the original paper that describes COX-2)

Winkler JD, Sung C-M, Hubbard WC, Chilton FH. Evidence for different mechanisms involved in the formation of lyso platelet-activating factor and the calcium-dependent release of arachidonic acid from human neutrophils. Biochem Pharmacol 44:2055-2066, 1992.

Triggiani M, Schleimer RP, Tomioka K, Hubbard WC, Chilton FH. Characterization of platelet-activating factor synthesized by normal and granulocyte-macrophage colony-stimulating factor-primed human eosinophils. Immunology 77:500-504, 1992.

Chilton FH, Cluzel M, Triggiani M. Recent advances in our understanding of the biochemical interactions between platelet-activating factor and arachidonic acid. Lipids 26:1021-1026, 1991.

Chilton FH, Patel M, Fonteh AN, Hubbard WC, Triggiani M. Dietary n-3 fatty acid effects on neutrophil lipid composition and mediator production: Influence of duration and dosage. J Clin Invest 91:115-122, 1993.

Fonteh AN, Chilton FH. Mobilization of different arachidonate pools and their roles in the generation of leukotrienes and free arachidonic acid during immunologic activation of mast cells. J Immunol 150:563-570, 1993.

Winkler JD, Sung C-M, Hubbard WC, Chilton FH. Influence of arachidonic acid on indices of phospholipase A2 activity in the human neutrophil. Biochem J 291:825-831, 1993.

Columbo M, Horowitz EM, Patella V, Kagey-Sobotka A, Chilton FH, and Lichtenstein LM. A comparative study of the effects of 1-acyl-2-acetyl-sn-glycero-3-phosphocholine and platelet-activating factor on histamine and leukotriene C4 release from human leukocytes. J Allergy Clin Immunol 92:325-333, 1993.

Fonteh AN, Winkler JD, Torphy TJ, Heravi J, Undem BJ and Chilton FH. Influence of isoproterenol and phosphodiesterase inhibitions on platelet-activating factor biosynthesis in the human neutrophil. J Immunol 151:339-350, 1993.

Shin M-H, Averill FJ, Hubbard WC, Chilton FH, Baroody FM, Liu MC, Naclerio RM. Nasal allergen challenge generates 1-0-hexadecyl-2-lyso-sn-glycero-3-phosphocholine. Am J Respir Crit Care Med 149:660-666, 1994.

Fonteh AN, Bass DA, Marshall LA, Seeds M, Samet JM, Chilton FH. Evidence that secretory phospholipase A2 plays a role in arachidonic acid release and eicosanoid biosynthesis by mast cells. J Immunol 152:5438-5446, 1994.

Stadel JM, Hoyle K, Naclerio RM, Roshak A, and Chilton FH. Characterization of phospholipase A2 from human nasal lavage. Am J Res Cell Mol Biol 11:108-113, 1994.

Bass DA, Seeds MC, Jones DF, Chilton FH, and Bauldry SA. Priming of phospholipases A2 of human neutrophils by tumor necrosis factor. Chest 105:99S-100S, 1994.

Diez E, Chilton FH, Stroup G, Mayer RJ, Winkler JD, Fonteh AN. Fatty acid and phospholipid selectivity of different phospholipase A2 enzymes using mammalian membrane as substrate. Biochem J 301:721-726, 1994.

Tramposch KM, Chilton FH, Stanley PL, Franson RC, Havens MB, Nettleton DO, Davern LB, Darling IM, Bonney RJ. Inhibitor of phospholipase A2 blocks eicosanoid and platelet activating factor biosynthesis and has topical anti-inflammatory activity. J Pharmacol Exp Ther 271:852-859, 1994.

Winkler JD, Sung C-M, Huang L, Chilton FH. CoA-independent transacylase activity is increased in human neutrophils after treatment with tumor necrosis factor I. Biochem Biophys Acta 1215:133-140, 1994.

Chilton FH, Fonteh AN, Sung C-M, Hickey TJ, Mayer RJ, Marshall LA, Heravi JD, and Winkler JD. Inhibitors of CoA-independent transacylase block the movement of arachidonate into 1-ether-linked phospholipids of human neutrophils. Biochemistry 34:5403-5410, 1995.

Samet JM, Fasano MB, Fonteh AN, and Chilton FH. Selective induction of prostaglandin G/H synthase I by stem cell factor and dexamethasone in mast cells. J Biol Chem 270:8044-8049, 1995.

Fonteh AN, Samet JM and Chilton FH. Regulation of arachidonic acid, eicosanoid, and phospholipase A2 levels in murine mast cells by recombinant stem cell factor. J Clin Invest 96:1432-1439, 1995.

Winkler JD, Fonteh AN, Sung C-M, Heravi JD, Nixon AB, Chabot-Fletcher M, Griswold D, Marshall LA, Chilton FH. Effects of CoA-independent transacylase inhibitors on the production of lipid inflammatory mediators. J Pharmacol Exp Ther 274:1338-1347, 1995.

Ely EW, Seeds MC, Chilton FH, and Bass DA. Neutrophil release of arachidonic acid, oxidants, and proteinases: causally related or independent. Biochim Biophys Acta 1258:135-144, 1995.

Triggiani M, Oriente A, Seeds MC, Bass DA, Marone G, Chilton FH. Migration of human inflammatory cells into the lung results in the remodeling of arachidonic acid into a triglyceride pool. J Exp Med 182:1181-1190, 1995.

Chilton FH, Fonteh AN, Surette ME, Triggiani M, Winkler JD. Control of arachidonate levels within inflammatory cells. Biochem Biophys Acta, 1299: 1-15, 1996.

Chilton FH, Averill FJ, Hubbard WC, Fonteh AN, Triggiani M, Lui M: Antigen-Induced generation of lyso-phospholipids in human airways. J Exp Med 183:2235-2245, 1996.

Chilton-Lopez T, Surette ME, Swan DD, Fonteh AN, Johnson MM, Chilton FH: Metabolism of gammalinolenic acid in human neutrophils. J Immunol 156:2941-2947, 1996.

Samet JA, Fonteh AN, Galli SJ, Tsai M, Fasano MB, Chilton FH. Alterations in arachidonic acid metabolism in mouse mast cells induced to undergo maturation in vitro in response to stem cell factor. J Allergy Clin Immunol 97:1329-1341, 1996.

Chilton, FH: Would the real biological role(s) for secretory, low molecular weight PLA2s please stand up. J Clin Invest 97:2161-2162, 1996. (editorial)

Surette ME, Winkler JD, Chilton FH: Relationship between arachidonate-phospholipid remodeling and apoptosis. Biochemistry 35:9187-9196, 1996.

Winkler JD, Surette ME, Fonteh AN, Chilton FH: The key roles played by CoA-independent transacylase in arachidonate movement and inflammatory cell functions. Pharmacol Rev Commun 8:19-27, 1996.

Bathon JM, Chilton FH, Hubbard WC, Towns MC, Solan MJ, Proud D: Mechanisms of prostanoid synthesis in cultured human synovial cells: cytokine-peptide synergism. Inflammation 20:537-554, 1996.

Winkler JA, Eris T, Sung C-M, Chabot-Fletcher M, Mayer RJ, Surette ME, Chilton FH: Inhibitors of coenzyme A-independent transacylase induce apoptosis in human HL-60 cells. J Pharmacology Exp Ther 279:956-966, 1996.

Johnson MM, Swan DD, Surette ME, Chilton-Lopez T, Fonteh AN, Chilton FH: The in vivo metabolism of gammalinolenic acid in healthy humans. J Nutrition 127:1435-1444, 1997.

Seeds MC, Jones DF, Chilton FH, Bass DA. Tumor necrosis factor primes two phospholipases A2 in human neutrophils. Biochim Biophys Acta 1389:273-284, 1998.

Surette ME, Chilton FH. The distribution and metabolism of arachidonate-containing phospholipids in cellular nuclei. Biochem J 330 (Pt 2):915-921, 1998.

Fonteh AN, Samet JM, Surette M, Reed W, Chilton FH. Mechanisms that account for the selective release of arachidonic acid from intact cells by secretory phospholipase A2. Biochim Biophys Acta 1393(2-3):253-266, 1998.

Johnson MM, Vaughn B, Triggiani M, Swan DD, Fonteh AN, Chilton FH. The role of triglyceride-containing lipid bodies in eicosanoid formation in human polymorphonuclear cells. Am J Respir Cell Mol Biol 21:253-258, 1999.

Surette ME, Fonteh AN, Bernatchez, C, Chilton FH. Perturbations in the control of cellular arachidonic acid levels block cell growth and induce apoptosis in HL-60 cells. Carcinogenesis 20:757-763, 1999.

Clay CE, Namen AM, Willingham MC, High KP, Kute TE, Trimboli AJ, Fonteh AN, Dawson PA, Chilton FH. Influence of J series prostaglandins on apoptosis and tumorigenesis of breast cancer cells. Carcinogenesis 20:1905-1911, 1999.

Trimboli T, Fonteh AN, Chilton FH. Influence of CoA-independent transacylase and cyclooxygenase inhibitors on the proliferation of breast cancer cells. Cancer Res 59:6171-6177, 1999.

Barham JB, Edens MB, Fonteh AN, Johnson MM, Easter L, Chilton FH. Addition of eicosapentaenoic acid to g-linolenic acid-supplemented diets prevents serum arachidonic acid accumulation in humans. J Nutr 130:1925-1931, 2000.

Fonteh AN, Atsumi G, LaPorte T, Chilton FH. Secretory phospholipase A2 receptor-mediated activation of cytosolic phospholipase A2 in murine bone marrow-derived mast cells. J Immunol 165:2773-2782 (2000).

Fonteh AN, Marion CR, Barham BJ, Edens, MB, Atsumi G, Samet JM, High KP, Chilton FH. Enhance mast cell survival: a novel function for some secretory PLA2 isotypes. J Immunol 167:4161-4171, 2001.

Clay CE, Namen AM, Trimboli AJ, Atsumi G, Fonteh AN, High KP, Chilton FH. The magnitude of PPARg activation is associated with important and seemingly opposite biological responses in breast cancer cells. J Invest Med 49: 413-420, 2001.

Clay CE, Atsumi G, High KP, Chilton FH. Early de novo gene expression is required for 15deoxy 12,14PGJ2 induced apoptosis in breast cancer cells. J Biol Chem 276: 47131-47135, 2001.

Clay CE, Thorburn J, Chilton FH, and High KP. 15-Deoxy 12,14PGJ2 induced apoptosis does not require PPARg in breast cancer cells. J Lipid Res 43:1818-1823, 2002.

Surette ME, Koumenis I, Edens M, Tramposch KM and Chilton FH. Evaluation of the Inhibition of Leukotriene Synthesis, Pharmacokinetics and Safety of a Novel Dietary Fatty acid Formulation in Healthy Subjects. Clin. Therapeutics 25: 972-979, 2003.

Surette ME, Koumenis I, Edens M, Tramposch KM, Clayton B, Bowton D and Chilton FH. A Randomized, Prospective, Placebo-Controlled, Parallel Group Trial on the Inhibition of Leukotriene Biosynthesis in Asthmatics by a Novel Dietary Fatty Acid Formulation. Clin Therapeutics 25: 948-971, 2003.

High KP, Sinclair J, Easter L, Chilton FH. Age associated changes in serum arachidonic acid and eicosapentaenoic acid levels in healthy adults. J. Health, Aging Nutri. 7:378-384, 2003.